Understanding how the amphibian chytrid fungus interacts with its environment and hosts

Presented by Dr Rebecca Webb, Melbourne University

• When: Thurs September 26th, 12 – 1 pm
• Where: Natural Resources Building, Large Lecture Theatre (EM2)

Abstract

The amphibian chytrid fungus, Batrachochytrium dendrobatidis, causes chytridiomycosis, a disease responsible for the decline or extinction of hundreds of amphibian species. Understanding the strengths and weaknesses of this fungal pathogen can inform mitigation measures. Here we develop stable genetic modification methods to customise a cell-line based in vitro chytrid infection model, and use fluorescent protein expression to allow real-time observation and quantification of chytrid growth in a host-like environment. As a case study, we examine the importance of host and fungal produced glutathione, and how this influences fungal stress tolerance and virulence.

Biography

Rebecca Webb loves all things frogs, but is more comfortable pipetting in the lab than catching frogs in the rain. Her research is focussed on the amphibian chytrid fungus, in particular understanding the mechanisms by which the pathogen tolerates stress encountered in the environment and when infecting the host.  This talk will explore how molecular biology tools and cell line infection models can help target stress tolerance and reduce the impact of the chytrid fungus. 

Contact:
Dr Ria Mukherjee : rmukherj@une.edu.au
David Perovic  david.perovic@une.edu.au